Remove a glass slide and cover slip and wipe clean with a towel if moist or dirty. With a pipette add a drop or two of saline onto the center of the glass slide. Use the surgical blade or a clean, single edge razor blade to scrape off some mucus and the upper layer of the skin, remove and place in the drop of saline on the slide. Cover with a cover slip and gently press down on the cover slip to spread the mucus and remove air bubbles. Add more saline if need and wipe excess saline from around the borders of the coverslip. Place side on the stage of the microscope and examined under low, medium and high dry power for ectoparasites and/or obvious structural abnormalities.
file: /Techref/other/pond/tilapia/wetmountskin.htm, 1KB, , updated: 2009/7/13 08:44, local time: 2024/9/1 03:18,
3.145.90.229:LOG IN
|
©2024 These pages are served without commercial sponsorship. (No popup ads, etc...).Bandwidth abuse increases hosting cost forcing sponsorship or shutdown. This server aggressively defends against automated copying for any reason including offline viewing, duplication, etc... Please respect this requirement and DO NOT RIP THIS SITE. Questions? <A HREF="http://piclist.com/techref/other/pond/tilapia/wetmountskin.htm"> HAMES Laboratory Method: Wet Mount Preparation of Skin for Microscopy</A> |
Did you find what you needed? |
![]() o List host: MIT, Site host massmind.org, Top posters @none found - Page Editors: James Newton, David Cary, and YOU! * Roman Black of Black Robotics donates from sales of Linistep stepper controller kits. * Ashley Roll of Digital Nemesis donates from sales of RCL-1 RS232 to TTL converters. * Monthly Subscribers: Gregg Rew. on-going support is MOST appreciated! * Contributors: Richard Seriani, Sr. |
Welcome to piclist.com! |
.